ABSTRACT
PURPOSE: Cell transplantation of myelin-producing exogenous cells is being extensively explored as a means of remyelinating axons in X-linked adrenoleukodystrophy. We determined whether 3,3',5-Triiodo-L-thyronine (T3) overexpresses the ABCD2 gene in the polysialylated (PSA) form of neural cell adhesion molecule (NCAM)-positive cells and promotes cell proliferation and favors oligodendrocyte lineage differentiation. MATERIALS AND METHODS: PSA-NCAM+ cells from newborn Sprague-Dawley rats were grown for five days on uncoated dishes in defined medium with or without supplementation of basic fibroblast growth factor (bFGF) and/or T3. Then, PSA-NCAM+ spheres were prepared in single cells and transferred to polyornithine/fibronectin-coated glass coverslips for five days to determine the fate of the cells according to the supplementation of these molecules. T3 responsiveness of ABCD2 was analyzed using real-time quantitative polymerase chain reaction, the growth and fate of cells were determined using 5-bromo-2-deoxyuridine incorporation and immunocytochemistry, respectively. RESULTS: Results demonstrated that T3 induces overexpression of the ABCD2 gene in PSA-NCAM+ cells, and can enhance PSA-NCAM+ cell growth in the presence of bFGF, favoring an oligodendrocyte fate. CONCLUSION: These results may provide new insights into investigation of PSA-NCAM+ cells for therapeutic application to X-linked adrenoleukodystrophy.
Subject(s)
Animals , Rats , ATP-Binding Cassette Transporters/metabolism , Adrenoleukodystrophy/genetics , Animals, Newborn , Bromodeoxyuridine , Cell Differentiation , Fibroblast Growth Factor 2/pharmacology , Fibronectins/metabolism , Immunohistochemistry , Neural Cell Adhesion Molecules/genetics , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction , Sialic Acids/metabolism , Stem Cells , Thyroid Hormones/metabolism , Triiodothyronine/pharmacologyABSTRACT
In this study, the effects of excessive iodine intake on free thyroixine index (FTI) were studied, and the results are presented in the following.<BR>FTI tended to decrease during the basal diet period without wakame seaweed and the decrease tended to continue further during the periods of TEST 1 (wakame seweed 10g/day) and TEST 2 (wakame seaweed 20g/day). The trend changed in the period of TEST 3 (wakame seaweed 40g/day), and FTI rose slightly. When the basal diet was resumed after completion of experimental diets, the level returned to the base line levels measured before the beginning of the experiment.